Fermentation production of L-methoinine and regulation of L-methionine biosynthesis in Corynebacterium glutamicum. III. L-methionine production by methionine analog-resistant mutants of Corynebacterium glutamicum.

Characterization of methionine export in Corynebacterium glutamicum.

Corynebacterium glutamicum is known for its effective excretion of amino acids under particular metabolic conditions. Concomitant activities of uptake and excretion systems would create an energy-wasting futile cycle; amino acid export systems are therefore tightly regulated. We have used a DNA microarray approach to identify genes for membrane proteins which are overexpressed under conditions ...

Enhanced production of L-lysine from Corynebacterium glutamicum by mutagenesis

Metabolic engineering of Corynebacterium glutamicum for L-serine production.

Although L-serine proceeds in just three steps from the glycolytic intermediate 3-phosphoglycerate, and as much as 8% of the carbon assimilated from glucose is directed via L-serine formation, previous attempts to obtain a strain producing L-serine from glucose have not been successful. We functionally identified the genes serC and serB from Corynebacterium glutamicum, coding for phosphoserine ...

[Regulation of methionine/cysteine biosynthesis in Corynebacterium glutamicum and related genomes].

Methionine is an essential amino acid and the universal N-terminal amino acid of proteins. The biosynthesis of methionine is extensively studied in various organisms that could be used in biotechnological production of methionine. Transcriptional regulation of the methionine synthesis in the Corynebacterium glutamicum genome is well studied. The McbR protein is a transcriptional regulator of me...

L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.

Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE sh...